Journal: Molecular Vision

Article Title: Coexpression of heparanase activity, cathepsin L, tissue factor, tissue factor pathway inhibitor, and MMP-9 in proliferative diabetic retinopathy

doi:

Figure Lengend Snippet: Pearson correlation coefficients.

Article Snippet: Subsequently, the sections were incubated for 60 min with mouse monoclonal anti-CD31 (ready-to-use; clone JC70A; Dako, Glostrup, Denmark), mouse monoclonal anti-CD45 (ready-to-use; clones 2B11+PD7/26; Dako), mouse monoclonal anti-cathepsin L antibody (1:400; ab6314, Abcam), mouse monoclonal antibody against TF (1:50; ab17375, Abcam), and rabbit polyclonal antibody against human TFPI (1:200; ab180619, Abcam).

Techniques: Activity Assay

Journal: Molecular Vision

Article Title: Coexpression of heparanase activity, cathepsin L, tissue factor, tissue factor pathway inhibitor, and MMP-9 in proliferative diabetic retinopathy

doi:

Figure Lengend Snippet: Comparisons of mean ± SD levels of cathepsin L, TF, TFPI and MMP-9 in vitreous fluid samples according to detection of heparanase enzymatic activity.

Article Snippet: Subsequently, the sections were incubated for 60 min with mouse monoclonal anti-CD31 (ready-to-use; clone JC70A; Dako, Glostrup, Denmark), mouse monoclonal anti-CD45 (ready-to-use; clones 2B11+PD7/26; Dako), mouse monoclonal anti-cathepsin L antibody (1:400; ab6314, Abcam), mouse monoclonal antibody against TF (1:50; ab17375, Abcam), and rabbit polyclonal antibody against human TFPI (1:200; ab180619, Abcam).

Techniques: Activity Assay

Journal: Molecular Vision

Article Title: Coexpression of heparanase activity, cathepsin L, tissue factor, tissue factor pathway inhibitor, and MMP-9 in proliferative diabetic retinopathy

doi:

Figure Lengend Snippet: Proliferative diabetic retinopathy epiretinal membranes. A : A negative control slide that was treated with an irrelevant antibody showed no labeling (original magnification 40X). B : Immunohistochemical staining for CD31 showing blood vessels positive for CD31 (original magnification 40X). Immunohistochemical staining for cathepsin L showing immunoreactivity in vascular endothelial cells (arrows) and stromal cells (arrowheads). C : Low power (original magnification 25X). D : High power (original magnification 40X).

Article Snippet: Subsequently, the sections were incubated for 60 min with mouse monoclonal anti-CD31 (ready-to-use; clone JC70A; Dako, Glostrup, Denmark), mouse monoclonal anti-CD45 (ready-to-use; clones 2B11+PD7/26; Dako), mouse monoclonal anti-cathepsin L antibody (1:400; ab6314, Abcam), mouse monoclonal antibody against TF (1:50; ab17375, Abcam), and rabbit polyclonal antibody against human TFPI (1:200; ab180619, Abcam).

Techniques: Negative Control, Labeling, Immunohistochemical staining, Staining